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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
10/02/2017 |
Data da última atualização: |
29/03/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
LOBO, F. P.; CINTRA, L. C.; VARELA, E. S.; ALVES, A. L.; SILVA, N. M. A. da; IANELLA, P.; PAIVA, S. R.; CAETANO, A. R. |
Afiliação: |
FRANCISCO PEREIRA LOBO, CNPTIA; LEANDRO CARRIJO CINTRA, CNPTIA; EDUARDO SOUSA VARELA, CNPASA; ANDERSON LUIS ALVES, CNPASA; NAIARA MILAGRES AUGUSTO DA SILVA, CENARGEN; PATRICIA IANELLA, CENARGEN; SAMUEL REZENDE PAIVA, SRI; ALEXANDRE RODRIGUES CAETANO, CENARGEN. |
Título: |
Genome Sequencing and de novo assembly of the South American Tiger Catfish (Pseudoplatystoma punctifer). |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: PLANT & ANIMAL GENOME CONFERENCE, 24., 2016, San Diego, CA. [Abstracts...]. San Diego: [s. n.], 2016. |
Páginas: |
Não paginado. |
Idioma: |
Inglês |
Notas: |
PAG 2016. Pôster P0481. |
Conteúdo: |
The South American Tiger Catfish (Pseudoplatystoma punctifer) is a freshwater fish species naturally found in the Amazon river basin with an important role in local community and commercial fishing. Recent efforts to domesticate and raise the species in aquaculture systems has led to >10-fold production increases in the last decade. Current production levels are >15,000mt/year. Recently stablished initiatives to structure genetic improvement programs for increasing productivity-associated traits could greatly benefit from using genomic tools for broodstock management and assisted genetic evaluations and breeding. Multiple shotgun libraries with two different insert sizes and multiple Nextera mate pair libraries with three different sizes were sequenced (2x160bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1.12pg = 1.095Gbp). Initial sequence assembly was performed with SOAPdenovo and generated 686.171 scaffolds spanning 1.41 Gbp (N50 scaffold length: 908Kbp, L50 scaffold count: 420). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species, which will be a valuable resource for basic biology studies, and marker detection/selection for use in genetic improvement and resource conservation activities. MenosThe South American Tiger Catfish (Pseudoplatystoma punctifer) is a freshwater fish species naturally found in the Amazon river basin with an important role in local community and commercial fishing. Recent efforts to domesticate and raise the species in aquaculture systems has led to >10-fold production increases in the last decade. Current production levels are >15,000mt/year. Recently stablished initiatives to structure genetic improvement programs for increasing productivity-associated traits could greatly benefit from using genomic tools for broodstock management and assisted genetic evaluations and breeding. Multiple shotgun libraries with two different insert sizes and multiple Nextera mate pair libraries with three different sizes were sequenced (2x160bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1.12pg = 1.095Gbp). Initial sequence assembly was performed with SOAPdenovo and generated 686.171 scaffolds spanning 1.41 Gbp (N50 scaffold length: 908Kbp, L50 scaffold count: 420). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species, which will be a valuable resource for basic biology studies, and marker detection/selection for use in genetic improvement and resource ... Mostrar Tudo |
Palavras-Chave: |
Bioinformática; Genômica; Sequenciamento de genoma. |
Thesaurus Nal: |
Bioinformatics; Genome assembly; Genomics; Sequence analysis. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140246/1/PAG-p0481.pdf
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Marc: |
LEADER 02464nam a2200301 a 4500 001 2063462 005 2023-03-29 008 2016 bl uuuu u01u1 u #d 100 1 $aLOBO, F. P. 245 $aGenome Sequencing and de novo assembly of the South American Tiger Catfish (Pseudoplatystoma punctifer).$h[electronic resource] 260 $aIn: PLANT & ANIMAL GENOME CONFERENCE, 24., 2016, San Diego, CA. [Abstracts...]. San Diego: [s. n.]$c2016 300 $aNão paginado. 500 $aPAG 2016. Pôster P0481. 520 $aThe South American Tiger Catfish (Pseudoplatystoma punctifer) is a freshwater fish species naturally found in the Amazon river basin with an important role in local community and commercial fishing. Recent efforts to domesticate and raise the species in aquaculture systems has led to >10-fold production increases in the last decade. Current production levels are >15,000mt/year. Recently stablished initiatives to structure genetic improvement programs for increasing productivity-associated traits could greatly benefit from using genomic tools for broodstock management and assisted genetic evaluations and breeding. Multiple shotgun libraries with two different insert sizes and multiple Nextera mate pair libraries with three different sizes were sequenced (2x160bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1.12pg = 1.095Gbp). Initial sequence assembly was performed with SOAPdenovo and generated 686.171 scaffolds spanning 1.41 Gbp (N50 scaffold length: 908Kbp, L50 scaffold count: 420). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species, which will be a valuable resource for basic biology studies, and marker detection/selection for use in genetic improvement and resource conservation activities. 650 $aBioinformatics 650 $aGenome assembly 650 $aGenomics 650 $aSequence analysis 653 $aBioinformática 653 $aGenômica 653 $aSequenciamento de genoma 700 1 $aCINTRA, L. C. 700 1 $aVARELA, E. S. 700 1 $aALVES, A. L. 700 1 $aSILVA, N. M. A. da 700 1 $aIANELLA, P. 700 1 $aPAIVA, S. R. 700 1 $aCAETANO, A. R.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
14/03/2008 |
Data da última atualização: |
15/05/2024 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
LIMA, L. M. de; VIEIRA, P. M. M. M.; VALENCIA, A. J.; CARNEIRO, R. M. D. G.; MARANHÃO, A. Q.; BRÍGIDO, M. de M.; GROSSI DE SÁ, M. F. |
Afiliação: |
LIZIANE MARIA DE LIMA, EMBRAPA ALGODÃO; PEDRO MANOEL M. M. VIEIRA, UNIVERSIDADE DE BRASÍLIA; ARNUBIO VALENCIA JIMÉNEZ; REGINA MARIA DECHECHI GOMES CARNEIRO, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; ANDRÉA QUEIROZ MARANHÃO, UNIVERSIDADE DE BRASÍLIA; MARCELO DE MACÊDO BRIGIDO, UNIVERSIDADE DE BRASÍLIA; MARIA FÁTIMA GROSSI DE SA, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA. |
Título: |
Caracterização de scFV selecionado contra proteínas de Meloidogyne incógnita a partir de uma biblioteca de Phage display. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. O algodão como oportunidade de negócios: resumos. Uberlândia: ABRAPA: AMIPA: Embrapa Algodão, 2007. |
Idioma: |
Português |
Thesagro: |
Algodão; Gossypium Hirsutum; Meloidogyne Incognita; Nematóide. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00811nam a2200217 a 4500 001 1182907 005 2024-05-15 008 2007 bl uuuu u01u1 u #d 100 1 $aLIMA, L. M. de 245 $aCaracterização de scFV selecionado contra proteínas de Meloidogyne incógnita a partir de uma biblioteca de Phage display. 260 $aIn: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. O algodão como oportunidade de negócios: resumos. Uberlândia: ABRAPA: AMIPA: Embrapa Algodão$c2007 650 $aAlgodão 650 $aGossypium Hirsutum 650 $aMeloidogyne Incognita 650 $aNematóide 700 1 $aVIEIRA, P. M. M. M. 700 1 $aVALENCIA, A. J. 700 1 $aCARNEIRO, R. M. D. G. 700 1 $aMARANHÃO, A. Q. 700 1 $aBRÍGIDO, M. de M. 700 1 $aGROSSI DE SÁ, M. F.
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